Microfluidics is also called laboratory on a chip (LOC). Since their development, microfluidic technologies have revolutionized the way for experimental biology and biomedical research, along with significant improvements in the fields of chemical synthesis, biofabrication, drug screening, and organ and tissue modeling. Importantly, microfluidics can integrate with POC testing to achieve high sensitivity, portability, and specificity. Recently, the application of microfluidic devices in the bioanalytical field has grown rapidly, such as in achieving the “sample-in/result-out” analysis mode, precisely tuning the size and pattern of nanoparticles for drug delivery, as well as discovering and screening new chemical entities.