How to Establish Pure Culture of Button Mushroom Using Potato D

• Posted by Mungus Mungus March 25, 2022 Filed in Health #penis envy mushrooms  64 views
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  How to Establish Pure Culture of Button Mushroom Using Potato Dextrose Agar on Petri Dish

  

  The process of establishing pure culture of Button Mushroom in a Petri dish is quite simple. Start by taking a slice of the mushroom penis envy mushrooms.

  Make sure that you use a scalpel blade to do this because a knife can introduce contaminants from the outer surface of the specimen. Then, place the slice sideways on a clean surface.

  Dip the cap of the petri plate into the liquid. Now, add two to three spores into the liquid. The spores should spread across the entire plate. Repeat these steps until you have a pure culture. Once you have obtained a sterile agar containing Button Mushroom spores, divide them among the remaining cultures.

  Then, mix 20 g of agar into a glass jar. You can use a 350 ml jar to use the medium. If you are using a molten agar, you can put it in a saucepan of water. This way, you can heat the medium to 80 degrees without any problem. After boiling the liquid, pour the agar powder last and let it sit for about twenty minutes.

  Next, prepare the mother culture plate for mycelium growth. After you have prepared the agar, you need to prepare the medium for the mushroom growth. Ensure that the plate is clean of bacteria and fungi and discard the rest. You must ensure that the mycelium is sterile by keeping the plate in a clean room.