The maximum common purpose for sterilization failure is lack of KGF Chapter 2 contact between the steam and the germs that you are trying to kill. Air normally movements to the lowest of the autoclave due to the fact it is heavier than steam and is then pressured out via the drain. It is vital that packs are located on their aspects to ensure that the air isn't trapped and can't break out. It is also less difficult for the air to be forced downwards between the packs than to go through the numerous layers of packs that have been positioned flat.
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